TABLE 1.
Adherence of different V. cholerae vaccine strains to the undifferentiated HT29-18N2 colonic epithelial cell line
| Strain | Relevant genotype and/or phenotype | Log CFU/wella
|
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|---|---|---|---|---|---|---|
| Inoculumb | Adherence afterc:
|
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| 0.5 h | 2 h | 4 h | 8 h | |||
| 81 | Δ(ctxA ctxB zot ace orfU cep), O1, El Tor, Ogawa mutant from C7258 | 6.94 ± 0.29 | 6.43 ± 0.15 | 7.81 ± 0.28 | 9.00 ± 0.39 | 8.90 ± 0.23 |
| 638 | hap::celA mutant from 81 | 7.27 ± 0.14 | 6.41 ± 0.08 | 8.00 ± 0.42 | 8.85 ± 0.35 | 9.34 ± 0.28 |
| JBK70 | Δ(ctxA ctxB), O1, El Tor, Inaba mutant from N16961 | 7.02 ± 0.44 | 6.88 ± 0.34 | 8.34 ± 0.22 | 9.36 ± 0.40 | 8.08 ± 0.27 |
| CVD103HgR | Δ(ctxA), hlyA::mer, O1, Classical, Inaba mutant from 569B | 7.67 ± 0.081 | 6.84 ± 0.20 | 8.74 ± 0.35 | 9.39 ± 0.33 | 8.53 ± 0.22 |
a
Mean log CFU/well from three independent experiments ± SEM.
b
Inoculum size of different V. cholerae strains to undifferentiated HT29-18N2 cells in three independent experiments.
c
The number of vibrios attached to HT29-18N2 on two replicate coverglasses from three independent experiments. No significant (P < 0.05) differences were observed among the cholera vaccine strains tested, at all times assayed.