Figure 3.

Maturation of fused micro 3D–cultured spheroids into elastic cartilage via rotating wall vessel (RWV) culture. (a) Schematic representation of micro 3D–cultured spheroids transferred into an RWV. (b) Representative image of tissue cultured in an RWV. Scale bars: 1 mm (c) Secretion level of melanoma inhibitory activity (MIA) and hyaluronic acid (HA) on a time course (n = 8). (d) Gene expression of AGGRECAN and ELASTIN against 18S in passage 2 chondroprogenitor cells, day 3 micro 3D–cultured spheroids and week 4 RWV–cultured cartilage (n = 4).

ns: not significant.

*p < 0.05. **p < 0.01.