Fig. 4.

Alterations in splenic metabolites between the two groups, and Spearman correlations in splenic metabolites, spleen cell populations, and gut microbes

A: Volcano plot showing splenic metabolite profiles between the CON and ABX groups. X-axis indicates the log2 transformed fold change in splenic metabolite abundance, and y-axis indicates the -log10 transformed P-value using the Wilcox test. Horizontal lines indicate P < 0.05. Red or blue dots indicate differential metabolites between the two groups. B: Differences in abundance of splenic differential metabolites between the two groups (statistical results are presented in Table S4). C: Spearman correlation analysis of splenic differential metabolites with percentage of splenic cell population or spleen weight. D: Spearman correlation analysis of differential species (average of abundance >0.1% in all samples) with splenic differential metabolites, percentage of splenic cell populations, or spleen weight. Values represent mean ± S.E.M. (CON: n = 8. ABX: n = 9). ^#P (FDR-corrected) < 0.05, ^##P (FDR-corrected) < 0.01, ^###P (FDR-corrected) < 0.001, ^####P (FDR-corrected) < 0.0001. *P < 0.05, **P < 0.01, ***P < 0.001. N.S.: not significant.