Figure 12.

Effects of IRF. Both histograms show the fluorophore’s inverse lifetime with ground truth shown by a red line. The bias in the peaks away from the ground truth arises from the limited amounts of data being used to learn the posterior shown. The corrected model (orange) reduces the histogram’s breadth compared with the uncorrected model (blue). We conclude from the small effects of correcting for the IRF that, predictably, the IRF may be less important under continuous illumination. By contrast, under pulsed illumination to be explored in the third companion article (54), the IRF will play a more significant role.