Figure 9.

System transition rates ${\lambda }_{esc}$ and FRET efficiencies ${ϵ}_{FRET}$ as functions of increasing donor crosstalk probability ${\phi }_{d1}$. To produce these plots, we generated synthetic data with excitation and escape rates as in Fig. 5. In each plot, the blue dots represent the median values (50% quantile), and the ends of the attached confidence intervals represent the 5 and 95% quantiles. Furthermore, the ground truths are shown with red horizontal lines. In (a), our two plots show system transition rates estimated by the BNP-FRET sampler when corrected and uncorrected for crosstalk. We show similar plots for FRET efficiencies in (b). In all plots, we see that, as donor crosstalk is increased, the confidence intervals grow (the posterior gets wider) and the estimates become unreliable after ${\phi }_{d1}>0.6$. In addition, as expected, if uncorrected for, the FRET efficiencies start to merge with increasing crosstalk due to most photons being detected in acceptor channel (labeled 1).