Figure 1.

Single-cell transcriptome analysis of buffalo prepubertal and pubertal testes

(A) Schematic diagram of the experimental workflow.

(B) Histomorphometric analysis (H&E staining) of prepubertal and pubertal buffalo testes. Scale bar, 100 μm.

(C) Dimension reduction presentation (by tSNE) of combined single-cell transcriptome data from prepubertal and pubertal testes (a total of 32,847 cells). Each point represents a single cell and is colored by sample.

(D) tSNE plot of prepubertal and pubertal testicular cell clusters defined by scRNA-seq analysis, colored according to cell type.

(E) Expression pattern of selected markers on the tSNE plot.

(F) Heatmap of normalized expression in DEGs (Wilcoxon rank sum test, p-value < 0.05) for each cell type. Labels indicate cell type (columns) and the number of genes (rows).

(G) GO terms (biological process) for identified cell types (p-value <0.05).