Figure 6.

Characterization of buffalo somatic lineages

(A) tSNE plot of somatic cell subsets in prepubertal and pubertal buffalo testes, colored by cell type (up) and sample (down).

(B) Re-clustering tSNE plot of SC lineages in buffalo testes, colored by cell type (left) and sample (right).

(C) Pseudotime trajectories of buffalo SCs subsets, coloring by cell type (left) and pseudotime value (right), respectively.

(D) Heatmap showing DEGs (q-value < 0.01) expression (rows) along the pseudotime (columns) axis.

(E) Feature plots, transcriptional trajectory plots, and pseudotime trajectory expression patterns of selected genes in Immature 1, Immature 2, and Mature clusters.

(F) Heatmap of differential genes in buffalo somatic cells (PTM, LC, M, EC, and NK) split by samples (PRE and PERI).

(G) tSNE plots showing gene relative expression levels of retinoic acid (up) and testosterone pathways (down).