Figure 5.

Effects of the soluble guanylate cyclase inhibitor, ODQ on NONOate-induced responses. 10 μM ODQ prevented the responses of both WT and Kir6.1^−/− vessels to NONOate. Representative traces of concentration-dependent NONOate responses after pre-incubation with 10 μM ODQ for 20 min in WT (A) and Kir6.1^−/− (B) popliteal lymphatics. Summary of absolute contraction amplitude (C) and frequency (D) compared before (“Krebs”) and after the treatment of ODQ. * Significant using paired Student’s t-test). Lymphatic contraction parameters were normalized to their respective values prior to ODQ treatment (E-G). Analysis of the effect of NONOate compared to the presence of 10 μM ODQ on normalized amplitude (E), normalized frequency (F) and change in EDD (G). Filled red rectangles indicate NONOate responses of WT vessels pretreated with ODQ (n=12) and open blue rectangles represent NONOate responses of Kir6.1^−/− pretreated with ODQ (n=8). In comparison, the response to NONOate only in each group of WT (n=24) and Kir6.1^−/− (n=8) is represented by filled black circles and open black circles respectively. All data are means ± SEM. * Significant difference in WT data from control (absence of NONOate). † Significant difference in Kir6.1^−/− data from control (absence of NONOate). ¥ (red) Significant difference between filled black circles and filled red rectangle data points. € (blue) Significant difference between open black circles and open blue rectangle data points.