Figure 3.
mRNA overexpression analysis of MEP and MVA pathway genes using qRT-PCR. (A) DXS, (B) DXR, (C) CMK, (D) IDI, (E) GPPS (F) LIS, (G) AAT, (H) HMGR, (I) FPS, (J) GAS, (K) GAO, and (L) COS. Gene expression levels were normalized to those of PP2A and their relative expression is shown. MEP, plastid 2-C-methyl-D-erythritol 4-phosphate; MVA, mevalonate; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; DXR, 1-deoxy-D-xylulose 5-phosphate reductoisomerase; CMK, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; IDI, isopentenyl-diphosphate isomerase; GPPS, geranyl pyrophosphate synthase; LIS, linalool synthase; AAT, acetoacetyl-CoA thiolase; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; FPS, farnesyl pyrophosphate synthase; GAO, germacrene A oxidase; GAS, germacrene A synthase; COS costunolide synthase; PP2A, protein phosphatase 2A.