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. 2022 Dec 26;14:187. doi: 10.1186/s13148-022-01415-3

Table 1.

Primer list

Name Sequence (5′-3′)
Inf-BDNF-pCpGL Fw GTGGATCCAGATCTTATTCCTCTGATACCCAG
Inf-BDNF-pCpGL Rv CTCCATGGACTAAGCTCCACCTTTTCAGTCAC
pCpGL-seq_Fw AAAGGAATTCCTGCAGGACTAGTG
pCpGL-seq_Rv TTGGCATCCTCCATGGACTAAGCT
bisBDNF_IV_forw1: GGGGGAGGATTAATTGAGTTAGTTTTG
bisBDNF_IV_forw2: TTTGTTGGGGTTGGAAGTGAAAAT
bisBDNF_IV_rev: ATA TAT ACT CCT TCT ATT CTA CAACAA
BDNF_IV_seq: ACAAAAAAATTTCATACTAA
Inf-DNMT1-Halo-Fw TCACTATAGGGCTAGCAGATGCCGGCGCGTACCGCC
Inf-DNMT1-Halo-Rv TCCGCGGTAGGAATTCGTCCTTAGCAGCTTCCTCCTCCTTTA
CpG87-mut-Fw TCTGGTAATTmCGTGCACTAGAG
CpG87-mut-Rv ATCAAAATTCAGCGCATTTAAAATG
CpG35_NEB-mut-Fw TGACAGCGCAmCGTCAAGGCAC
CpG35_NEB-mut-Rv TATGATACCTCCGCTGCC
CpG39_NEB-mut-Fw CATATGACAGmCGCACGTCAAGGC
CpG-39_NEB-mut-Rv ATACCTCCGCTGCCTCGA
CpG87-NEB-Control-Fw TCTGGTAATTCGTGCACTAGAG
CpG87-NEB-Control-Rv ATCAAAATTCAGCGCATTTAAAATG

Gene-specific sequence is highlighted in the primers used for cloning