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. Author manuscript; available in PMC: 2022 Dec 27.
Published in final edited form as: Cell Rep. 2022 Dec 6;41(10):111759. doi: 10.1016/j.celrep.2022.111759

Figure 3. IRS2/PI3K/ GSK3β axis sustains MYC expression.

Figure 3.

(A and B) Relative expression of MYC protein in IRS2−/− SUM-159 cells (A) or IRS2−/− SUM-159 cells with restored IRS2 expression (B).

(C and D) Relative levels of MYC expression and pS62-MYC phosphorylation in MG132-treated IRS2−/− SUM-159 cells (C) or IRS1−/−, IRS2−/− SUM-159 cells with restored IRS2 expression (D).

(E) IRS1−/−, IRS2−/− SUM-159 cells expressing IRS2 were serum starved and stimulated with each ligand (50 ng/mL) for the time periods indicated.

(F) Irs1−/−, Irs2−/− PyMT cells expressing Irs2-WT were stimulated with insulin (500 ng/mL) for the time periods indicated.

(G) Irs1−/−, Irs2−/− PyMT cells expressing Irs2-WT or Irs2-Y5F were stimulated with IGF1 (50 ng/mL) for the time periods indicated.

The data shown in all graphs represent the mean ± SD of three or more independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.

See also Figure S4.