Fig. 3.

Effects of GP17 on the Hif1α-BNIP3 and SIRT1/2-FOXO3A signalling pathways and thus on the mitochondrial autophagy signalling pathway in rats with MCAO/R injury. A and B The protein bands of HIF1α-BNIP3 in ischemic brain sections were examined by western blot analysis. C and D The relative expression levels of Hif1α and BNIP3, respectively, were quantified and analysed by using Gel-Pro analyser software. E and F The protein bands of SIRT1/2/3 in the ischemic brain sections were examined by western blot analysis. G and H The protein bands of NAMPT, FOXO3A, and MnSOD in the ischemic brain sections were examined by western blot analysis. I–K The relative expression levels of SIRT1, SIRT2 and SIRT3 were quantified and analysed by using Gel-Pro analyser software. L–N The relative expression levels of NAMPT, FOXO3A, and MnSOD were quantified and analysed by using Gel-Pro analyser software. Mean values ± SEM (n=3–6). Statistical comparisons were performed as follows: one-way ANOVA with Dunnett’s multiple comparisons test for (C, D, K, L, and N); unpaired t test for (I, J, M, and K); ^*P<0.05, ^**P<0.01 versus MCAO/R group; ^#P<0.05, ^##P<0.01 versus Sham group