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. 2022 Dec 27;20:622. doi: 10.1186/s12967-022-03830-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effects of GP17 on autophagic vesicles, autophagic lysosomes, LC3-B expression and the Beclin1-LC3-P62 signalling pathway in OGD/R-subjected SH-SY5Y cells were partly reversed by the inhibitor 3-MA. A MDC staining from all SH-SY5Y cell groups as measured by a fluorescence microscope; scale bar = 100 μm. B Triple staining of autophagosome-lysosomes (red), mitochondria (green) and nuclei (blue) in OGD/R-subjected SH-SY5Y cells, as measured by a fluorescence microscope; scale bar = 20 μm. C Triple staining of LC3-B protein (green), mitochondria (red) and nuclei (blue) in OGD/R-subjected SH-SY5Y cells, as located and measured by a fluorescence microscope; scale bar, 20 μm. D The protein bands of LC3-B, Beclin1 and P62 were examined by western blot analysis in OGD/R-subjected SH-SY5Y cells. E–G. The expression levels of Beclin1 and P62, and the ratio LC3-B II and I in OGD/R-subjected SH-SY5Y cells were quantified and analysed by using Gel-Pro analyser software. The data presented as Mean values ± SEM. Statistical comparisons were performed as follows: unpaired t test for (E, F, and G); ^#P < 0.05, ^##P < 0.01 versus the control group; ^*P < 0.05 versus the OGD/R model group; ^&P < 0.05 versus the OGD/R + GP17 group