Fig. 5.

Effects of GP17 on mitochondria-related indicators and autophagy. The Beclin1-LC3-P62 signalling pathway in OGD-induced SH-SY5Y cells was suppressed by the inhibitors AGK-7 and 2-ME. A Mitochondrial fluorescence images of GP17 to assess the MMP in OGD/R-subjected SH-SY5Y cells, as measured by JC-1 assay with a fluorescence microscope; scale bar, 100 μm. B Representative images of mitochondria stained with MitoTracker Red-CMXRos obtained with a fluorescence microscope; scale bar, 200 μm. C Triple staining of LC3-B protein (green), mitochondria (red) and nuclei (blue) in OGD/R-subjected SH-SY5Y cells, as located and measured by a fluorescence microscope; scale bar = 20 μm. D The protein bands of LC3, Beclin1 and P62 were examined by western blot analysis in OGD/R-subjected SH-SY5Y cells. E–G The expression levels of Beclin1 and P62, and the ratio LC3-B II and I in OGD/R-subjected SH-SY5Y cells were quantified and analysed by using Gel-Pro analyser software. The data presented as Mean values ± SEM. Statistical comparisons were performed as follows: unpaired t test for (E, F, and G); ^#P < 0.05, ^##P < 0.01 versus the control group; ^*P < 0.05 versus the OGD/R model group; ^&P < 0.05 versus the OGD/R +  1 group