Figure 2.

Arrangement of samples and controls in a 96‐well plate format and sample addition to the 384‐well white plate. (A) A 96‐well plate can accommodate 90 patient samples with three positive controls (suggested as three concentrations of a serology standard [red]) and three negative prepandemic serum controls (blue). (B) After arranging samples in a 96‐well format, the serum samples are diluted in the phosphate‐buffered saline containing bovine serum albumin and Tween 20 and then transferred to a 384‐well plate containing either S sensors or N sensors in every other well and row in the plate to avoid spillover signal. Each set of 90 samples will be run on four total 384‐well white plates (two replicates [rep] for each sensor).