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Schematic of Alternate Protocol 2 for running the split‐luciferase antibody assay in a point‐of‐care setting. Initially, the protein biosensors are lyophilized in PCR tubes and then reconstituted with phosphate‐buffered saline (PBS) at the start of the assay. Then, finger prick blood is transferred to the sensor PCR tube with a microcapillary pipette. After incubation, diluted substrate is added, and the sample is centrifuged. Luminescence is measured on a handheld luminometer.
