Extended Data Fig. 12. Effect of IL-2(WT) versus IL-2(V) on LCMV-specific CD8⁺ T cells during chronic infection.

a, Experimental design for data in panels b and c. Mice chronically infected with LCMV (> 40 days post infection) were untreated or treated with IL-2 wild-type (IL-2(WT)) for 5 days, and CD25 expression was checked on PD-1negative and PD-1⁺ CD8⁺ T cells in the spleen. b, Representative FACS plots of CD25 expression. c, Summary plots of CD25 expression after IL-2(WT) and IL-2(V) treatments. d, Experimental design for data in panels e and f. Mice chronically infected with LCMV were untreated, treated with IL-2(WT) or treated with IL-2 variant (IL-2(V)) for 6 days. Expression of IL-2 receptors (CD25, CD122, and CD132) on LCMV-specific CD8⁺ T cells in the spleen were examined. e, f, Representative histograms (e) and summary plots (f) of expression of IL-2 receptors on D^bGP33⁺ CD8⁺ T cells after indicated treatments. Results were pooled from 2 experiments with 2–3 mice per group in each experiment. Data are presented as mean and SD (c, f) with p values. Statistical comparisons were performed using one-way ANOVA with Tukey’s multiple comparison test. Untx, untreated.