Figure 7.

Comparison of O174L wildtype and mutant nucleotide and protein sequence and the effects of observed mutations on the wild-type ASFV PolX protein structure. Alignment of ASFV O174L wildtype and mutant nucleotide sequence (A) and protein sequence (B) including structural information from the literature [34]. Catalytic sites (red box), mutation site (blue box), amino acids forming the 5’-binding pocket (green box) and altered amino acids (magenta letters) are highlighted. The nucleotide alignment was done using MAFFT v7.4506 and the protein alignment using Clustal W in Geneious. (C) X-ray structure of wild-type ASFV PolX in complex with nicked DNA (PDB accession: 5HRI) [34]. Positions with altered sequence in the mutant are coloured in cyan and positions that are missing in the mutant are coloured in magenta. The illustration was prepared with PyMol (Schrödinger, Inc.).