TABLE 3.
Threshold of IL-1β-induction in human whole blooda
| Stimulus | IL-1β induction threshold (pg/ml)b |
|---|---|
| Borrelia lysate | |
| B. burgdorferi | 1,000 |
| B. garinii | 1,000 |
| B. afzelii | 1,000 |
| LPS | |
| S. enterica serovar Enteritidis | 10 |
| K. pneumoniae | 10 |
| S. enterica serovar Abortusequi | 10 |
| E. coli O26:B6 | 1,000 |
| B. pertussis | 1,000 |
| P. aeruginosa | 10,000 |
| V. cholerae | 10,000 |
a
Human whole blood diluted 1:5 in RPMI was incubated in the presence of Borrelia lysate (0.1 ng/ml to 100 μg/ml) or various endotoxins (1 pg/ml to 10 μg/ml) in 10-fold serial dilutions for 24 h at 37°C.
b
The IL-1β level in the cell-free supernatant was measured by ELISA. Data represent the lowest concentration of the given stimulus where blood from all four healthy donors released significant amounts of IL-1β.