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. 2021 Dec 30;13(1):60–76. doi: 10.1080/21505594.2021.2018767

Figure 5.

Figure 5.

Cytosolic mtDNA triggers activation of pro-apoptotic caspase-1 in A. hydrophila-infected HKM. (a) HKMs pre-incubated with Z-YVAD-FMK, 4-PBA, YCG063 or transfected with mtDNA, DNase I, heat-inactivated DNase I were infected with or without A. hydrophila and caspase-1 activity studied at 12 h p.i. (b) HKMs incubated with Z-YVAD-FMK were infected with A. hydrophila and HKM apoptosis studied at 24 h p.i. Vertical bars represent mean ± SE (n = 3). Asterisk indicates significant difference between indicated groups (*p < 0.05). HKM, uninfected HKM; HKM+B, HKM infected with A. hydrophila; HKM+Z-YVAD-FMK+B, HKM pre-incubated with Z-YVAD-FMK infected with A. hydrophila; HKM+4-PBA+B, HKM pre-incubated with 4-PBA infected with A. hydrophila; HKM+YCG063 + B, HKM pre-incubated with YCG063 infected with A. hydrophila; HKM+mtDNA, HKM transfected with mtDNA; HKM+DNase I + B, HKM transfected with DNase I infected with A. hydrophila; HKM+heat-inactivated DNase I + B, HKM transfected with heat-inactivated DNase I infected with A. hydrophila.