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. Author manuscript; available in PMC: 2023 Dec 22.
Published in final edited form as: Cell. 2022 Dec 22;185(26):4986–4998.e12. doi: 10.1016/j.cell.2022.11.033

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rat anti-FLAG antibody BioLegend Cat #637301;
RRID:AB_1134266
Horseradish peroxidase-conjugated anti-rat secondary antibody Cell Signaling Technology Cat #7077S
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
1,2-dipalmitoyl-sn-glycero-3-phosphate (PA) Avanti Polar Lipids Cat #830855P
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) Avanti Polar Lipids Cat #850757
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (POPG( Avanti Polar Lipids Cat #840457
3,3’-dithiobis(sulfosuccinimidyl propionate) (DTSSP) Thermo Fisher Scientific Cat #21578
3X FLAG peptide Pepmic Custom synthesis, no identifier
Anti-FLAG M2 resin Sigma-Aldrich Cat #A2220
BHI medium HIMEDIA Cat #N210
Hemin chloride Sigma Cat #3741
Penicillin-Streptomycin Gibco Cat #15070063
Poly-L-lysine Sigma-Aldrich Cat #P8920
Phusion High-Fidelity DNA polymerase Thermo Fisher Scientific Cat #F-530S
Polycarbonate membrane Avanti Polar Lipids Cat #610005
Trypsin Promega Cat #90057
Critical Commercial Assays
Novex ECL chemiluminescence kit Thermo Fisher Scientific Cat #WP20005
Membrane lipid strips Echelon Bioscience Cat #P-6002
Deposited Data
Subtomogram average of C. reinhardtii IFT-A obtained from anterograde trains McCafferty et al., 202240 EMDB: EMD-26791
Subtomogram average of C. reinhardtii IFT-A obtained from anterograde trains Jordan et al., 201819 EMDB: EMD-4304
Composite subtomogram average of an assembled C. reinhardtii IFT train at the ciliary transition zone van den Hoek et al., 202218 EMDB: EMD-15261
Composite cryo-EM map of Leishmania tarentolae IFT-A state 1 This paper EMDB: EMD-28866
Composite cryo-EM map of Leishmania tarentolae IFT-A state 2 This paper EMDB: EMD-28867
Atomic model of Leishmania tarentolae IFT-A state 1 This paper PDB: PDB 8F5O
Atomic model of Leishmania tarentolae IFT-A state 2 This paper PDB: PDB 8F5P
Chemical crosslinking mass spectrometry data obtained for Tetrahymena thermophila IFT-A McCafferty et al., 202240 MassIVE/ProteomeXchange: PXD032818
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Leishmania tarentolae strain P10 Jena Bioscience Cat #LT-101
Bacteria: Escherichia coli DH5α New England Biolabs Cat #C2987H
Oligonucleotides
LtIFT43_Fw1
CCTTGCCACCAGATCTGCCATGTCCACGACTTCTATGACTGCC		 Sigma N/A
LtIFT43_Rv1
CCGGAGCCTCTAGACTTCGAAACCTGCTTGGATG		 Sigma N/A
Recombinant DNA
Plasmid: pLEXSY-hyg2.1 Jena Bioscience Cat #EGE-1310hyg
Plasmid: pLEXSY-hyg2.1_LtIFT43_Flag This paper Addgene: Cat #194433
Software and Algorithms
AlphaFold2 Jumper et al., 202170 https://www.deepmind.com/
ChimeraX v1.4 Pettersen et al., 202169 https://www.rbvi.ucsf.edu/chimerax/
Clustal Omega v1.2.2 Sievers et al., 201174 http://www.clustal.org/omega/
Coot v.0.9.8.3 Brown et al., 201571 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
cryoSPARC v3.3.2 Punjani et al., 201766 https://cryosparc.com/
DeepEMhancer Sanchez-Garcia et al., 202162 https://github.com/rsanchezgarc/deepEMhancer
Prism v9 Graphpad Software, USA https://www.graphpad.com/
phenix.combine_focused_maps v1.18.2–3874 Liebschner et al, 201968 https://www.phenix-online.org/
phenix.molprobity v1.18.2–3874 Chen et al., 201073 https://www.phenix-online.org/
phenix.real_space_refine v1.18.2–3874 Afonine et al., 201872 https://www.phenix-online.org/
RELION 4.0 Zivanov et al., 201867 https://www3.mrc-lmb.cam.ac.uk/relion/
SBGrid Morin et al., 201365 https://sbgrid.org/
SerialEM v.3.8.5 Schorb et al., 201964 https://bio3d.colorado.edu/SerialEM/
SEQUEST Thermo Fisher Scientific https://www.thermofisher.com
Other
Aclar coverslips Electron Microscopy Sciences Cat #50425–25
Carbon-coated copper grids Electron Microscopy Sciences Cat #CF200-Cu
R2/1, 200 mesh gold, QUANTIFOIL grids Electron Microscopy Sciences Cat #220210