Hybridization analysis of chromosomal DNA from different Brachyspira species. Chromosomal DNA from different Brachyspira species was digested with AseI, and the fragments were separated by agarose electrophoresis and transferred to a nylon membrane. Hybridization was performed using a 0.95-kb ClaI-EcoRI fragment from plasmid pISM1236 as described in Materials and Methods and shown in Fig. 2. Lanes: 1, B. pilosicoli P43/678; 2, B. hyodysenteriae B204; 3, B. murdochii 155-20; 4, B. intermedia PWS/A; 5, strain C-1; 6, B. innocens B256. Molecular masses (in kilobases) are given on the left.