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Purification of the B. hyodysenteriae beta-hemolysin
| Fraction | Hemolytic activitya
|
% Protein | HU per mg of protein | |
|---|---|---|---|---|
| (HU/μl) | HU per mg (dry wt) | |||
| Unconcentrated crude | <1 | 20 | 1 | 2,000 |
| Concentrated crude | 11 | 2,200 | 28.4 | 11,957 |
| CEb | 17 | 3,400 | 11 | 30,910 |
HU, hemolytic units.
CE, capillary electrophoresis fraction (purified).
