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. 2001 Feb;69(2):712–718. doi: 10.1128/IAI.69.2.712-718.2001

FIG. 3.

FIG. 3

Screening for fibronectin-binding proteins according to the Western affinity blotting technique described previously (1). Lanes: 1, surface proteins released from an LiCl extract of S. aureus strain DU5883 (pFNBA4) (17) used as positive control; 2, proteins released from uninduced culture of E. coli M15 harboring pREP4 and pIP1818; 3, 66-kDa protein resulting from purification on Ni-nitrilotriacetic acid resin (Qiagen) of the proteins released from an IPTG-induced culture of E. coli M15 harboring pREP4 and pIP1818; 4: proteins released from an LiCl extract of S. caprae isolate 96007.