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Demonstration by immunofluorescence and confocal microscopy of the presence of asialo-GM1 on the surface of MDCK cells growing in Transwells. After the addition of asialo-GM1 in DMSO to the cells, followed by incubation, washing, and staining with antibody to asialo-GM1, ganglioside was seen in cells treated with asialo-GM1 in DMSO but not in cells treated with DMSO only. Magnification, ×400.
