Skip to main content
. 2022 Dec 19;45(12):911–922. doi: 10.14348/molcells.2022.0130

Fig. 3. Phosphorylation is required for structural loading of N in virus-like particle (VLP).

Fig. 3

(A) The 293 cells were transfected with E, M, S and empty vector (EMS) or EMS with wt N expression vector (EMS + N) or EMS with N-L223/227E expression vector (EMS + N-L223/227E) and VLPs were subjected to TEM analysis (left panels). Scale bars = 200 nm. The VLP sizes of EMS (93 ± 16 nm), EMS + N (121 ± 22 nm) and EMS-N-L223/227E (87 ± 18 nm) were determined by TEM images (right). *P < 0.05 by t-test. (B) Cell lysate (left) for VLP production in 293 cells and purified VLP (right) were subjected to western blot analysis to determine HA-tagged E, M, S, and flag-tagged N or N-L223/227E. S2, S2 subunit of S; WCL, whole cell lysate.