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. 2022 Dec 12;45(12):886–895. doi: 10.14348/molcells.2022.2031

Fig. 4. Chb-M’ induces the suppression of survivin and apoptosis in MRT cells in vitro and in vivo.

Fig. 4

(A) Downregulation of survivin in Chb-M’-treated KP-MRT-YM cells. Cells were treated with Chb-M’ or dimethyl sulfoxide (DMSO; control). Forty-eight hours after treatment, cell lysates were prepared and subjected to immunoblotting. (B) Quantification of apoptotic cells. Apoptotic cells were defined as Annexin V+/7-AAD−cells (n = 3). Data are represented as the mean ± SEM. *P < 0.05, by two-tailed Student’s t-test. (C) Relative densitometric quantification of human apoptosis array spots in Chb-M’-treated MRT cells compared to the control. Cells were treated with DMSO or 1 μM Chb-M’ for 48 h and lysed for human apoptosis array. (D) Representative microscopic images of tumor histology. These tumors were extracted from MRT model mice treated with Chb-M’ (n = 6) or DMSO as control (n = 6). Results obtained from staining and immunohistochemical staining with anti-human survivin antibody are shown (original magnification ×4 and ×20 [insets]).