TABLE 2.
Distribution of CD44, CD24, and ALDH1A1 expression assessments from pathologist and Definiens readings [N of cores (%)].
| Pathologist |
Definiens |
|||||
| Tissue marker/Tissue type | All cores | Normal TDLUs | Benign lesions | All cores | Normal TDLUs | Benign lesions |
| CD44 | ||||||
| Categorical | ||||||
| Stroma | ||||||
| 0 | 112 (34.46) | 55 (31.43) | 57 (38.00) | 62 (19.02) | 35 (20.00) | 27 (17.88) |
| > 0 <1 | 61 (18.77) | 38 (21.71) | 23 (15.33) | 40 (12.27) | 24 (13.71) | 16 (10.60) |
| ≥1–≤10 | 66 (20.31) | 31 (17.71) | 35 (23.33) | 100 (30.67) | 57 (32.57) | 43 (28.48) |
| >10–≤50 | 55 (16.92) | 33 (18.86) | 22 (14.67) | 91 (27.91) | 46 (26.29) | 45 (29.80) |
| >50 | 31 (9.54) | 18 (10.29) | 13 (8.67) | 33 (10.12) | 13 (7.43) | 20 (13.25) |
| Epithelium | ||||||
| 0 | 30 (15.63) | 16 (15.84) | 14 (15.38) | 5 (1.91) | 5 (3.62) | 0 |
| > 0 <1 | 6 (3.13) | 4 (3.96) | 2 (2.20) | 4 (1.53) | 1 (0.72) | 3 (2.42) |
| ≥1–≤10 | 34 (17.71) | 20 (19.80) | 14 (15.38) | 45 (17.18) | 24 (17.39) | 21 (16.94) |
| >10–≤50 | 80 (41.67) | 40 (39.60) | 40 (43.96) | 98 (37.40) | 57 (41.30) | 41 (33.06) |
| >50 | 42 (21.88) | 21 (20.79) | 21 (23.08) | 110 (41.98) | 51 (36.96) | 59 (47.58) |
| Binary, with 1% cut-off | ||||||
| Stroma | ||||||
| <1 | 173 (53.23) | 93 (53.14) | 80 (53.33) | 102 (31.29) | 59 (33.71) | 43 (28.48) |
| ≥1 | 152 (46.77) | 82 (46.86) | 70 (46.67) | 224 (68.71) | 116 (66.29) | 108 (71.52) |
| Epithelium | ||||||
| <1 | 36 (18.75) | 20 (19.80) | 16 (17.58) | 9 (3.44) | 6 (4.35) | 3 (2.42) |
| ≥1 | 156 (81.25) | 81 (80.20) | 75 (82.42) | 253 (96.56) | 132 (95.65) | 121 (97.58) |
| Binary, with 10% cut-off | ||||||
| Stroma | ||||||
| ≤10 | 239 (73.54) | 124 (70.86) | 115 (76.67) | 202 (61.96) | 116 (66.29) | 86 (56.95) |
| >10 | 86 (26.46) | 51 (29.14) | 35 (23.33) | 124 (38.04) | 59 (33.71) | 65 (43.05) |
| Epithelium | ||||||
| ≤10 | 70 (36.46) | 40 (39.60) | 30 (32.97) | 54 (20.61) | 30 (21.74) | 24 (19.35) |
| >10 | 122 (63.54) | 61 (60.40) | 61 (67.03) | 208 (79.39) | 108 (78.26) | 100 (80.65) |
| CD24 | ||||||
| Categorical | ||||||
| Stroma | ||||||
| 0 | 188 (58.39) | 105 (59.32) | 83 (57.24) | 40 (12.12) | 33 (18.03) | 7 (4.76) |
| > 0 <1 | 74 (22.98) | 34 (19.21) | 40 (27.59) | 59 (17.88) | 36 (19.67) | 23 (15.65) |
| ≥1–≤10 | 43 (13.35) | 30 (16.95) | 13 (8.97) | 145 (43.94) | 72 (39.34) | 73 (49.66) |
| > 10–≤50 | 13 (4.04) | 7 (3.95) | 6 (4.14) | 66 (20.00) | 30 (16.39) | 36 (24.49) |
| >50 | 4 (1.24) | 1 (0.56) | 3 (2.07) | 20 (6.06) | 12 (6.56) | 8 (5.44) |
| Epithelium | ||||||
| 0 | 56 (28.14) | 30 (28.30) | 26 (27.96) | 0 | 0 | 0 |
| > 0 <1 | 41 (20.60) | 24 (22.64) | 17 (18.28) | 3 (1.10) | 2 (1.35) | 1 (0.80) |
| ≥1–≤10 | 34 (17.09) | 19 (17.92) | 15 (16.13) | 71 (26.01) | 43 (29.05) | 28 (22.40) |
| >10–≤50 | 42 (21.11) | 24 (22.64) | 18 (19.35) | 123 (45.05) | 71 (47.97) | 52 (41.60) |
| >50 | 26 (13.07) | 9 (8.49) | 17 (18.28) | 76 (27.84) | 32 (21.62) | 44 (35.20) |
| Binary, with 1% cut-off | ||||||
| Stroma | ||||||
| <1 | 262 (81.37) | 139 (78.53) | 123 (84.83) | 99 (30.00) | 69 (37.70) | 30 (20.41) |
| ≥1 | 60 (18.63) | 38 (21.47) | 22 (15.17) | 231 (70.00) | 114 (62.30) | 117 (79.59) |
| Epithelium | ||||||
| <1 | 97 (48.74) | 54 (50.94) | 43 (46.24) | 3 (1.10) | 2 (1.35) | 1 (0.80) |
| ≥1 | 102 (51.26) | 52 (49.06) | 50 (53.76) | 270 (98.90) | 146 (98.65) | 124 (99.20) |
| Binary, with 10% cut-off | ||||||
| Stroma | ||||||
| ≤10 | 305 (94.72) | 169 (95.48) | 136 (93.79) | 244 (73.94) | 141 (77.05) | 103 (70.07) |
| >10 | 17 (5.28) | 8 (4.52) | 9 (6.21) | 86 (26.06) | 42 (22.95) | 44 (29.93) |
| Epithelium | ||||||
| ≤10 | 131 (65.83) | 73 (68.87) | 58 (62.37) | 74 (27.11) | 45 (30.41) | 29 (23.20) |
| >10 | 68 (34.17) | 33 (31.13) | 35 (37.63) | 199 (72.89) | 103 (69.59) | 96 (76.80) |
| ALDH1A1 | ||||||
| Categorical | ||||||
| Stroma | ||||||
| 0 | 94 (27.33) | 56 (29.47) | 38 (24.68) | 32 (10.85) | 20 (12.27) | 12 (9.09) |
| > 0 <1 | 73 (21.220) | 42 (22.11) | 31 (20.13) | 19 (6.44) | 10 (6.13) | 9 (6.82) |
| ≥1–≤10 | 80 (23.26) | 37 (19.47) | 43 (27.92) | 143 (48.47) | 82 (50.31) | 61 (46.21) |
| >10–≤50 | 64 (18.60) | 34 (17.89) | 30 (19.48) | 88 (29.83) | 42 (25.77) | 46 (34.85) |
| >50 | 33 (9.59) | 21 (11.05) | 12 (7.79) | 13 (4.41) | 9 (5.52) | 4 (3.03) |
| Epithelium | ||||||
| 0 | 180 (90.00) | 98 (90.74) | 82 (89.13) | 0 | 0 | 0 |
| >0 < 1 | 12 (6.00) | 9 (8.33) | 3 (3.26) | 2 (0.74) | 1 (0.68) | 1 (0.82) |
| ≥1–≤10 | 6 (3.00) | 1 (0.93) | 5 (5.43) | 71 (26.39) | 41 (27.89) | 30 (24.59) |
| >10–≤50 | 2 (1.00) | 0 | 2 (2.17) | 166 (61.71) | 86 (58.50) | 80 (65.57) |
| >50 | 0 | 0 | 0 | 30 (11.15) | 19 (12.93) | 11 (9.02) |
| Binary, with 1% cut-off | ||||||
| Stroma | ||||||
| <1 | 167 (48.55) | 98 (51.58) | 69 (44.81) | 51 (17.29) | 30 (17.40) | 21 (15.91) |
| ≥1 | 177 (51.45) | 92 (48.42) | 85 (55.19) | 244 (82.71) | 133 (81.60) | 111 (84.09) |
| Epithelium | ||||||
| <1 | 192 (96.00) | 107 (99.07) | 85 (92.39) | 2 (0.74) | 1 (0.68) | 1 (0.82) |
| ≥1 | 8 (4.00) | 1 (0.93) | 7 (7.61) | 267 (99.26) | 146 (99.32) | 121 (99.18) |
| Binary, with 10% cut-off | ||||||
| Stroma | ||||||
| ≤10 | 247 (71.80) | 135 (71.05) | 112 (72.73) | 194 (65.76) | 112 (68.71) | 82 (62.12) |
| >10 | 97 (28.20) | 55 (28.95) | 42 (27.27) | 101 (34.24) | 51 (31.29) | 50 (37.88) |
| Epithelium | ||||||
| ≤10 | 198 (99.00) | 108 (100.00) | 90 (97.83) | 73 (27.14) | 42 (28.57) | 31 (25.41) |
| >10 | 2 (1.00) | 0 | 2 (2.17) | 196 (72.86) | 105 (71.43) | 91 (74.59) |
TDLU, terminal duct lobular unit.