Figure 2.

The expression of receptivity markers is dysregulated in infertile organoids after hormone treatments. (A) Schematic of hormone treatments for organoids. Passaged fertile and infertile organoids were cultured for 4 days in ExM to reform organoid structure before being subjected to E2 (to mimic proliferative phase) or E2+MPA+cAMP treatment (to mimic receptive phase). (B, C) After hormone treatments, organoid cells were collected for RT-qPCR analysis to determine the expression of receptivity markers. Expression levels were normalized to 18S (n=7-9 biological replicates). All data were presented as mean ± SEM. *P<0.05, **P<0.01, ns, no significant difference.