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. 2022 Dec 14;13:1067648. doi: 10.3389/fendo.2022.1067648

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Copyright © 2022 Zhou, Barton, Cui, Santos, Yang, Stern, Kieu, Teh, Ang, Lucky, Sgroi, Ye and Dimitriadis

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Examination of candidate proteins identified by mass spectrometry. (A) Immunoblot validation of MUC5AC expression in fertile and infertile IOF. Equal amounts of protein were loaded for examination (n=6-11 biological replicates). (B, C) qPCR examination of the production of candidate proteins in organoid cells. Top 6 significantly decreased (B) and increased (C) proteins ranked by fold change in infertile IOF compared to fertile IOF were selected for analysis. Gene expression levels were normalized to 18S (n=5 biological replicates). All data were presented as mean ± SEM. *P<0.05, **P<0.01, ns, no significant difference; IOF, Intra-organoid fluid.