Table 1.

Experiment design by RSM

Level	Factor
	OD	Host Cell	IPTG (mM)
1	0.6	BL 21	0.2
2	1.2	SHuffle	0.5
3	1.8	Nico	0.8

Response Surface Methodology (RSM) with 3 Factor
Run	Factor 1: OD	Factor 2: Host Cell	Factor 3: IPTG
1	0.6	BL 21 (1)	0.2
2	0.6	BL 21 (1)	0.8
3	1.2	BL 21 (1)	0.5
4	1.8	BL 21 (1)	0.2
5	1.8	BL 21 (1)	0.8
6	0.6	SHuffle (2)	0.5
7	1.2	SHuffle (2)	0.2
8	1.2	SHuffle (2)	0.8
9	1.2	SHuffle (2)	0.5
10	1.2	SHuffle (2)	0.5
11	1.2	SHuffle (2)	0.5
12	1.8	SHuffle (2)	0.5
13	0.6	Nico (3)	0.2
14	0.6	Nico (3)	0.8
15	1.2	Nico (3)	0.5
16	1.8	Nico (3)	0.8
17	1.8	Nico (3)	0.2