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. Author manuscript; available in PMC: 2022 Dec 28.
Published in final edited form as: Nat Genet. 2022 Feb 3;54(2):170–179. doi: 10.1038/s41588-021-00993-x

Extended Data Fig. 10. GATA3 influenced hematopoietic cell transformation in vitro and homing in vivo.

Extended Data Fig. 10

a, IL3-independent growth of Ba/F7 cells transduced with GATA3 alone, JAK2R683G alone, GATA3 with JAK2R683G, or empty vector control. All experiments were performed in triplicates (n=3, p-value = 0.0000915 by 2-sided 2-way ANOVA test). Ba/F7 cells with GATA3 and JAK2R683G were treated with or without 10 ng/ml TSLP. b, Zebrafish xenograft model of GM12878 cell migration. GM12878 WT and A/A cells were labeled with Vybrant DiO and then injected to 2 dpf Tg(kdrl:mcherry) transgenic embryos. 3dpf injected embryos were imaged under microscope for bright field and fluorescence. Red color shows the blood vessel of fish embryos. Green color shows GM12878 cells. Yellow arrows indicates GM12878 cells homing to the caudal hematopoietic tissue (CHT). Scale bar = 200 uM. Experiments were repeated twice independently with similar results. c, Quantitative analysis of GM12878 cell homing to the caudal hematopoietic tissue (CHT) (n = 11, p-value=3.924e-08, two-sided unpaired t-test). Data are presented as mean +/− SEM.