Fig. 4. Experimental validation of predicted recombinases for novel target sites.

a Alignment of half-sites from novel target sites with their respective CRL half-sites. Conserved bases are shown with a “.”. b PCR-based recombination assay of predicted recombinase clones (PRL single clone) and their respective CRL. Check marks indicate that the PCR products contained the edited sequence, while crosses indicate that recombination could not be confirmed by sequencing. Dashes were placed where no sequence verification was performed. PRL: predicted recombinase library, CRL closest recombinase library to novel target site in terms of target site similarity. Source data are provided as a Source Data file.