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. 2022 Dec 9;54(12):2188–2199. doi: 10.1038/s12276-022-00891-0

Fig. 7. Scheme of SCON mouse generation in 1–2 crossing steps and specific target gene application.

Fig. 7

a SCON mouse generation in 1-cell-stage embryos via microinjection or electroporation. The embryos either (i) carry inducible CreER alleles or (ii) are wild-type. b The F0 founder mice are screened for SCON insertion in the target gene (gene X). Mice carrying a SCON insertion are crossed with each other (i) or are crossed with a CreER-bearing mouse (ii). c The resulting F1 offspring may contain genotypes ready for in vivo cKO studies (i). d The F1 offspring that carry a gene X-SCON allele as well as the CreER allele are crossed with each other to obtain experiment-ready cKO mice in the F2 generation (ii). e Illustration of the insertion of SCON in single-exon genes. f Illustration of the insertion of SCON into genes that express alternatively spliced transcripts. SCON can be inserted in the alternatively spliced exon, which would lead to the protein truncations produced by isoform 1, while isoform 2 is intact. g Illustration of the insertion of SCON in large exons (>1000 bp), which leads to various sizes of truncations.