FIG. 5.

Induction of NO by commercial LTA preparations from C3H/HeJ peritoneal macrophages. C3H/HeJ macrophages were treated with increasing concentrations of S. aureus, B. subtilis, and S. sanguis LTA in the presence of 30 U of IFN-γ/ml for 20 h. Culture supernatants were then collected for a nitrite assay as described in Materials and Methods. Data represent averages of triplicate samples ± standard errors of the mean. Experiments were repeated three times. None of the three types of commercial LTA alone induced detectable NO production (data not shown).