FIG 1.

The Sia signaling network impacts attachment of P. aeruginosa to surfaces. (A) The Sia signaling network regulates SiaD diguanylate cyclase activity. In response to an unknown environmental signal, SiaA dephosphorylates SiaC. SiaC then binds SiaD, activating SiaD DGC activity. SiaB is a kinase that competitively binds SiaC and rephosphorylates it. Once rephosphorylated, SiaC will no longer bind SiaD, and DGC activity is shut off. Figure created in BioRender.com (29). (B) Static biofilm formation of sia mutant strains. Biofilm biomass produced by each strain was measured by crystal violet staining and normalized to wild type (PAO1). Presented as mean and standard deviation. N, 3 biological replicates, *P < 0.05. (C) Adherence of sia mutant strains to glass. Cells were incubated on a glass coverslip, rinsed and attached cells were immediately quantified by microscopy. Presented as mean and standard deviation. N, 3 biological replicates, *, P < 0.05.