FIG 4.
Planktonic levels of cell-associated Psl are not impacted by DGCs than SiaD. (A) Representative immunoblot for Psl produced by DGC transposon insertion mutants, extracted from midlog planktonic cells (OD600nm = 0.5). RNAP served as a loading control. (B) Quantification of cell-free and (C) cell-associated Psl production calculated using blots in 4A. Psl band intensity was normalized to RNAP levels and then compared to the wild-type control (MPAO1). Presented as mean and standard deviation. N, 3 biological replicates, *, P < 0.05.