FIG 5.
Overproduction of c-di-GMP causes an increase in cell-associated Psl levels. (A) C-di-GMP levels of cells overexpressing the heterologous cyclase Ml1419c in P. aeruginosa. C-di-GMP was extracted from midlog planktonic cells (OD600nm = 0.5). Presented as mean and standard deviation. N, 5 biological replicates, *, P < 0.05. (B) Representative immunoblot for Psl produced by cells overexpressing siaD or ml1419c, extracted from midlog, planktonic cells (OD600nm = 0.5). RNAP served as a loading control. (C) Quantification of relative Psl production calculated using blots in 5B. Psl band intensity was normalized to RNAP levels and then compared to the wild-type vector control (PAO1 VC) cell-associated Psl. Presented as mean and standard deviation. N, 3 biological replicates, *, P < 0.05, VC=vector control.