Figure 2.

CD40L cells (CDLCs), lymphoid stromal cells (LSCs), or CD40LCs + LSCs were co-cultured with CD19⁺ B-cells in CK2 (Table 1) and tested for their potential to support different B-cell subsets over time in vitro (2D)

(A) Schematic overview of the studied 2D co-culture conditions.

(B) Absolute number of viable CD19⁺ B cells over time.

(C) Flow cytometry results of one of the analyzed donors, illustrating the differences between the tested supporting cell combinations on the co-cultured B-cells at day 14.

(D) Fluorescent images of the added supporting cell types in the co-cultures (blue = DAPI, red = phalloidin, scale bars represent 200 μm).

(E) B cell subsets (total cell counts) within each culture well of the absolute number of cells on days 4, 7, 11 and 14, of the six B-cell subsets.

(F) Production of IgX (IgM, IgG, and IgA) in the culture supernatant on days 4, 7, 11 and 14.

(E and F) Data showing the mean ± SD (n = 3). ∗ = p > 0.05, ∗∗ = p > 0.01, ∗∗∗ = p > 0.001, ∗∗∗∗ = p > 0.0001.