Table 2.
Description of commonly used prompt fluorescence (JIP-test) parameters.
| Fluorescence parameters | Description | References |
|---|---|---|
| F 0 = ABS/CS 0 | initial fluorescence obtained in a dark adapted sample | Strasser et al., 2004 |
| F L = F 150 | fluorescence at 150 μs after illumination of a dark adapted sample | Oukarroum et al., 2007 |
| F K = F 300 | fluorescence at 300 μs after illumination of a dark adapted sample | Strasser et al., 2004 |
| F J = F 2ms | fluorescence at 2 ms after illumination of a dark adapted sample | Strasser et al., 2004 |
| F I = F 30ms | fluorescence at 30 ms after illumination of a dark adapted sample | Strasser et al., 2004 |
| F M = F P | maximum fluorescence after illumination of a dark adapted sample | Strasser et al., 2004 |
| V L = (F 150–F 0)/(F M –F 0) | relative variable fluorescence at 150 μs after illumination of a dark adapted sample | Oukarroum et al., 2007 |
| V K = (F 300−F 0)/(F M −F 0) | relative variable fluorescence at 300 μs after illumination of a dark adapted sample | Strasser et al., 2004 |
| V J = (F 2ms − F 0)/(F M − F 0) | relative variable fluorescence at 2 ms after illumination of a dark adapted sample | Strasser et al., 2004; Strasser et al., 2010 |
| V I = (F 30ms − F 0)/(F M − F 0) | relative variable fluorescence at 30 mμs after illumination of a dark adapted sample | Strasser et al., 2004; Strasser et al., 2010 |
| V K /V J | efficiency of electron flow from OEC to PSII reaction centres | Strasser et al., 2004; Strasser et al., 2010 |
| M 0 = 4 (F 300− F 0)/(F M − F 0) | approximated initial slope of the fluorescence transient, expressing the rate of RCs’ closure | Strasser et al., 2004 |
| φ Po = TR 0/ABS = F V /F M = (F M −F 0)/F M | maximum quantum yield of PSII photochemistry | Strasser et al., 2004 |
| ψ o = ET 0/TR 0= (F M – F 2ms )/(F M – F 0) = 1 – V J | probability that a trapped exciton moves an electron into the electron transport chain beyond QA | Strasser et al., 2004; Strasser et al., 2010 |
| δ Ro = RE 0/ET 0= (F M – F 30ms )/(F M – F 2ms ) | probability that an electron from the intersystem electron carriers is transferred to reduce end electron acceptors at the PSI acceptor side | Strasser et al., 2010 |
| ψ REo = ΔV IP = ψ Eo × δ Ro | total efficiency of electron transport from PSII to PSI | Strasser et al., 2010; Bussotti et al., 2020 |
| RC/ABS = γ RC /(1 – γ RC ) = φ Po (V J /M 0) | QA reducing RCs per PSII antenna chlorophyll | Strasser et al., 2004 |
| RC/CS 0 = φ Po (V J /M 0) (ABS/CS 0) | density of active RCs (QA reducing RCs) per cross section at point 0 | Strasser et al., 2004 |
| PI ABS = RC/ABS × φ Po /(1 – φ Po ) × ψ Eo /(1 – ψ Eo ) | performance index (potential) for energy conservation from photons absorbed by PSII to the reduction of intersystem electron acceptors | Strasser et al., 2004 |
| PI total = RC/ABS × φ Po /(1 – φ Po ) × ψ Eo /(1 – ψ Eo ) × δ Ro /(1 – δ Ro ) | performance index (potential) for energy conservation from photons absorbed by PSII to the reduction of PSI end electron acceptors | Strasser et al., 2010 |