Figure 3.

pO₂ steers canonical signaling pathways that direct naive B cell differentiation pathways (A-D) gMFI of (A) BCL-6 (n = 6) (B) IRF4 (n = 6) (C) PNA+ (n = 6) (D) CD95 (n = 6) (E) CD86 (n = 6) (F) NFκB active subunit p65 (n = 9) (G) c-Myc (n = 9) (H-I) gMFI of (H) tSTAT6 and pSTAT6 and (I) tSTAT3 and pSTAT3 over time in culture (n = 6) and (left panels) representative histogram overlays of pSTAT and tSTAT expression on day 5 of culture (J) gMFI of PAX5, BLIMP1 and %XBP-1s+ in BLIMP1hi cells (n=6) on day 7 (A-J) Bars represent means of biological replicates each composed of two technical replicates of 2 (H-J) or (A-G) 3 independent experiments (A-I) Differences in gMFI were determined using mixed-effects analysis using Tukey’s test for multiple comparisons. (J) Differences in gMFI were determined using repeated measures one-way ANOVA using Tukey’s tests for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.