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Properly orientating the grid bars helps to maximize the area within a square in which you can place a lamella. (A) Example of a cryo-FIB AutoGrid with the notch marked. The opposite side of the AutoGrid is shown with a marking that corresponds to the notch, which is visible during the clipping process. (B) Alignment of the grid bars as seen in a stereoscope without sample and (C) with sample in the grid clipping chamber.
