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. 2022 Nov 5;12(21):e4544. doi: 10.21769/BioProtoc.4544

Table 3. Milling parameters in AutoTEM Cryo.

Lamella thickness (defined as “Pattern Offset”) (µm) Front Width Overlap (µm) Rear Width Overlap (µm) Milling Current (nA) Pattern Type DCM Rescan Interval (s)
Rough Milling 1.0 1.5 1.0 1.0 Rectangle 120
Medium Milling 0.8 0.65 0.5 1.0 CCS 90
Fine Milling 0.6 0.35 0.1 0.5 CCS 60
Finer Milling 0.4 0.05 0.05 0.3 CCS 30
Polishing 1 0.15 N/A N/A 0.03 CCS 30
Polishing 2 0 N/A N/A 0.01 CCS 10