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. 2022 Dec 27;6(3):e202201592. doi: 10.26508/lsa.202201592

Figure 2. Transduction efficiency and auditory brainstem response after eGFP expression viral vector injection.

Figure 2.

(A, B, C) 2 wk after injection of eGFP expression viral vectors, whole-mount dissection was performed. Cochleae were stained with Myo7a (magenta) for labeling hair cells and imaged for native eGFP (cyan). Auditory brainstem responses were measured in the injected ear and uninjected contralateral ear at 4 and 10 wk of age. Only AAV2/2 eGFP w/WPRE-injected mice showed hearing impairment. Scale bar represents 100 μm. (D, E) Transduction efficiency in inner hair cells and outer hair cells. Data are means ± SEM. N mice per experimental condition are in parentheses.