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Schematic illustration of Tmc1 exonic structure, illustrating the position of siRNAs designed in this study. Blue exons were targeted by siRNAs designed by siSPOTR. siRNAs labeled in magenta were not successfully cloned into the pFBAAVmU6mcsCMVeGFP expression plasmid and tested for knockdown efficiency with RT–PCR. Inset: siRNA sequences.
