Figure 7.

Quiescent culture reverts human HSPCs to quiescence and maintains their primitive state after NHEJ-based editing

(A) Experimental design for editing human HSPCs.

(B) Total number of genome-edited CD34⁺CD38⁻CD45RA⁻ cells 2 days after electroporation (mean ± SD, n = 3–6 from independent cell cultures).

(C) Frequency of CD34⁺EPCR⁺CD90⁺ cells among genome-edited CD34⁺CD38⁻CD45RA⁻ cells 2 days after electroporation (mean ± SD, n = 3–6 from independent cell cultures).

(D) Frequency of cells in G0 phase derived from genome-edited CD34⁺CD38⁻CD45RA⁻ cells 2 days after electroporation (mean ± SD, n = 3 from independent cell cultures).

(E) Number of colonies formed by genome-edited CD34⁺CD38⁻CD45RA⁻CD90⁺ cells (mean ± SD, n = 3 per group).

(F) Total number of genome-edited CD34⁺CD38⁻CD45RA⁻CD90⁺ cells 7 days after electroporation (mean ± SD, n = 3 from independent cell cultures).

(G) Frequency of CD34⁺CD45⁻ cells among genome-edited CD34⁺CD38⁻CD45RA⁻CD90⁺ cells 7 days after electroporation (mean ± SD, n = 3 from independent cell cultures).