Fig. 2.

Haplotype networks for Sparicotyle chrysophrii from the Mediterranean built using 36 partial mitochondrial cox1 sequences (see Table 1 and Supplementary Table S2 for details). A Statistical parsimony network constructed with TCS 1.21 software under 95% connection limits. The connecting lines indicate the maximum number of steps connecting parsimoniously two haplotypes. The small black circles represent alternative unsampled haplotypes. B Comparative median-joining network built with PopART v.1.7. The hatch marks on the connecting lines indicate the number of the mutational steps among the haplotypes. A-B Pie chart sizes are proportional to haplotype frequency; haplotype frequency scale is indicated by colourless semicircles. Circle colours indicate the locations of origin for the distinct haplotypes as follows: yellow/red: Adriatic Sea, off Croatia; green, off Sète, France; dark blue, off Bouzedjar, Algeria; light blue, off Annaba, Algeria. Abbreviations: H1–H12, haplotype IDs.