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. 2022 Dec 14;13:1021370. doi: 10.3389/fimmu.2022.1021370

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Copyright © 2022 Voss, Howarth, Wittenborn, Hummelgaard, Juul-Madsen, Kastberg, Pedersen, Jensen, Papanastasiou, Vorup-Jensen, Weyer and Degn

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B cells harboring a GC block contribute to the PC lineage in a GC sufficient environment. (A) Schematic overview of the mixed bone marrow chimera setup. Lethally irradiated CD45.1/1 recipients were reconstituted with CD45.2/2 564Igi BM, WT CD45.1/2 BM and either Bcl-6^flx/flx (Cre-, orange, n=7) or Aicda-Cre Bcl-6^flx/flx (Cre+, light orange, n=8) (both CD45.2/2). (B) dsDNA IgG2c TRIFMA. (C) dsDNA total Ig TRIFMA. (D) Flow cytometric analysis of B cell frequencies (B220⁺ of live, singlets). (E) CD4 frequencies (CD4 of live, singlets). (F) CD8 frequencies (CD8 of live, singlets). (G) GCB frequencies (CD95^hi CD38^lo of B cells). (H) PB/PC frequencies (CD138^hi of live, singlets). (I) Representative bivariate plots with gates for Bcl-6^flx/flx chimeras. (J) Representative bivariate plots with gates for Aicda-Cre Bcl-6^flx/flx chimeras. (K) Ratio of CD45.2+ of GCB to CD45.2+ of total B cells. (L) Ratio of CD45.2+ of PBs/PCs to CD45.2+ of total B cells. The results are obtained from a single experiment with the number of mice given above. Bar graphs show mean ± SD. ns = p≥0.05, *p < 0.05, ***p < 0.001.