a, By activating mdg4 RNAi from embryonic to adult stage, the survival rates were measured by raising flies on CrPV- or IIV-6-containing food for 20 days. sh-white flies served as controls. b, By activating mdg4 RNAi from embryonic to adult stage, the survival rates were measured after infecting adult files with different viruses with a single meal. c, RT-qPCR to quantify the fold change of DCV mRNA in sh-mdg4 flies at different time points after one-time infection, relative to sh-white controls based on the two-tailed t-test. d, RT-qPCR to quantify the fold change of DCV mRNA in sh-mdg4 flies on day 6 after one-time infection, relative to sh-white controls based on the two-tailed t-test. The bars in panel c and d report mean ± standard deviation for three biological replicates. Comparison of survival curves was completed using a Cox proportional-hazards model for panels a and b.