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. Author manuscript; available in PMC: 2022 Dec 28.
Published in final edited form as: Nat Genet. 2022 Nov 17;54(12):1933–1945. doi: 10.1038/s41588-022-01214-9

Extended Data Fig. 8 |. Relish activation renders hosts protection from virus infection.

Extended Data Fig. 8 |

a, By activating Relish RNAi from embryonic to adult stage, the survival rates were measured by raising flies on CrPV- or IIV-6-containing food for 20 days. sh-white flies served as controls. b, RT-qPCR to quantify the fold changes of DCV mRNA in sh-relish flies on day 6 after one-time infection, relative to sh-white controls based on the two-tailed t-test. c, RT-qPCR to quantify the fold changes of DCV mRNA in sh-relish flies on day 6 after one-time infection, relative to sh-white controls based on the two-tailed t-test. The bars in panel B and C report standard deviation for three biological replicates. d, By activating Relish RNAi only at adult stage, the survival rates were measured by raising flies on DCV- or FHV-containing food for 20 days. sh-white flies served as controls. e, RT-PCR experiments to monitor the amount of DCV in adult dcr-2 mutant (dcr-2L811fsX) flies after one-time infection. Two independent biological replicates were performed. f, RT-qPCR to quantify relish expression upon mdg4 depletion in fly pupae. Data are normalized to rp49 (RpL32) expression; the bars report mean ± standard deviation for 2 biological replicates. Expression was compared using a two-tailed t-test. Comparison of survival curves was completed using a Cox proportional-hazards model for panels a and d.